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Arch Iran Med March. 2020;23(3): 163-168.
  Abstract View: 160
  PDF Download: 231

Original Article

Prevalence of Mycobacterium abscessus among the Patients with Nontuberculous Mycobacteria

Saman Ayoubi 1 * ORCID logo, Jafar Aghajani 1, Poopak Farnia 2, Parissa Farnia 1, Jalaledin Ghanavi 1, Ali Akbar Velayati 1

1 Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Biotechnology, School of Advanced Technology in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Background: Considering the importance of the increasing incidence of non-tuberculous mycobacteria, especially Mycobacterium abscessus worldwide, we conducted a study to evaluate the incidence of these diseases in our area. The aim of this study was to evaluate the prevalence of M. abscessus in patients with non-tuberculous mycobacteria.

Methods: This descriptive study was performed on 18,083 samples isolated from patients with non-tuberculous mycobacteria during 2011-2017 at the Mycobacteriology Research Center (MRC), Tehran, Iran. To identify the Mycobacterium species, a 439 bp fragment of the IS6110 gene was first amplified using primers TB1 and TB2. Samples with a negative polymerase chain reaction (PCR) result were analyzed to investigate non-tuberculosis mycobacteria (NTM), especially M. abscessus using the RFLP method.

Results: Of the 18,083 samples, 5513 (30.49%, 95% CI, 12.95) strains of Complex Tuberculosis and 236 (1.31%, 95% CI, 1.84) strains of NTM were identified. The mean age of the patients with NTM was 18 years, and most of them were male. The most commonly identified species in this study were M. abscessus type Ι 32 (13.56%, 95% CI, 18.36) and M. abscessus type II 13 (5.51%, 95%CI, 20.04).

Conclusion: In this study, we observed a high prevalence of Mycobacterium abscessus type 1 in patients. As the treatment protocol for non-TB mycobacteria is different from M. abscessus complex, the diagnosis of these species as soon as possible will be significant for physicians.

Keywords: Mycobacterium abscessus, Nontuberculous mycobacteria, Polymerase chain reaction, Prevalence
Cite this article as: Ayoubi S, Aghajani H, Farnia P, Farnia P, Ghanavi J, Velayati AA. Prevalence of Mycobacterium abscessus among the patients with nontuberculous mycobacteria. Arch Iran Med. 2020;23(3):163–`68.
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Submitted: 11 May 2019
Accepted: 25 Sep 2019
ePublished: 01 Mar 2020
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