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Arch Iran Med. 2020;23(9): 586-592.
doi: 10.34172/aim.2020.68

Scopus ID: 85091810804
  Abstract View: 237
  PDF Download: 261

Original Article

Occult Hepatitis C Virus Infection among Hemodialysis Patients: An Iranian Experience

Sedigheh Taherpour 1 ORCID logo, Davod Javanmard 1,2, Masood Ziaee 1* ORCID logo

1 Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
2 Department of Medical Microbiology, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
*Corresponding Author: Masood Ziaee, MD; Infectious Diseases Research Center, Birjand University of Medical Sciences, Ghafari Ave., Birjand, 9717853577, Iran. Tel: +98-9151613942, Fax: +98-5632433004, Email: dr.masood.ziaee@bums.ac.ir

Abstract

Background: Patients with chronic kidney failure and those undergoing chronic hemodialysis (CHD) treatment are at high risk of infection with hepatitis C virus (HCV). The incidence of occult HCV infection (OCI) in CHD remains controversial and the real burden of HCV in this population may be affected by the rate of OCI. This study evaluates the molecular assessment of OCI in CHD in an Iranian population.

Methods: All subjects on CHD in the South Khorasan province of Iran were invited for participation in the study. Whole blood samples were taken and serological, clinical, and demographic information was recorded. HCV-RNAs were checked in serum and peripheral blood mononuclear cells (PBMCs) using an in-house semi-nested PCR assay. Viral load was determined using a real-time PCR-based quantification kit. Sequencing was performed to determine genotypes.

Results: Overall, 120 cases participated in the study; 57.5% were male and the rest were female. In serum samples, no positive case was found for HCV-RNA. In PBMC samples, 2/120 (1.6%) were positive for HCV-RNA (95% CI, 0.002 to 0.059); the mean age of OCI positive cases was 37.5 ± 19.2 years which was significantly lower than OCI negative cases (P = 0.026). Only one case had detectable viral load which was 49 IU/mL. The only HCV genotype identified was 1a.

Conclusion: This study showed that there is a risk of OCI among CHD patients; the very low and undetectable viral loads of OCI warrant further follow-up molecular testing for earlier diagnosis and treatment in the era of DAA.

Keywords: Dialysis, Genotype, Hemodialysis, Hepatitis C virus, Occult HCV
Cite this article as: Taherpour S, Javanmard D, Ziaee M. Occult hepatitis C virus infection among hemodialysis patients: an Iranian experience. Arch Iran Med. 2020;23(9):586–592. doi: 10.34172/aim.2020.68.
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Submitted: 14 Dec 2019
Accepted: 09 Mar 2020
ePublished: 01 Sep 2020
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