Arch Iran Med. 2017;20(3): 0.
PMID: 28287811
Scopus ID: 85014702168
  Abstract View: 2031
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Original Article

Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes

Mahmood Naderi, Abdolreza Pazouki, Ehsan Arefian, Seyed Mahmoud Hashemi, Fatemeh Jamshidi-Adegani, Omid Gholamalamdari, Sara Soudi, Kayhan Azadmanesh, Siamak Mirab Samiee, Shahin Merat, Mohammad Gholami Fesharaki, Mahdieh Mondanizadeh, Mohammad Vasei*
*Corresponding Author: Email: soleim_m@modares.ac.ir


BACKGROUND: Expression of miR-122 is highly specific to hepatocytes of the liver.  This miRNA is involved in lipid hemostasis of the tissue; however, there is no comprehensive understanding of its function in lipid hemostasis. 

MATERIALS AND METHODS: Since hepatocytes are responsible for part of Triacylglycerol (TAG) synthesis in the body, we hypothesized that miR-122, as the most abundant miRNA in the tissue, might regulate TAG metabolism by targeting key enzymes that are involved in its production pathway. A systematic computational analysis of putative targets of miR-122 identified CTDNEP1 and LPIN1 genes in the TAG pathway. We used dual-luciferase reporter assay, quantitative RT-PCR as well as western blot to confirm the repressive effect of miR-122 on CTDNEP1 and LPIN1 in TAG pathway. 
RESULTS: Real time PCR on liver needle biopsies with hepatosteatosis showed that miR-122 is up-regulated in hepatosteatosis. Surprisingly, the protein and RNA level of identified targets of miR-122 are also up-regulated in clinical samples, probably as a disproportionate feedback response to the high level of miR-122. 
CONCLUSION: Our findings suggest that up-regulation of miR-122 can trigger the compensatory response of LPIN1 and CTDNEP1 in hepatosteatosis.
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