Arch Iran Med. 2018;21(7):289-295.
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Original Article

The Effects of Probiotic Supplementation on Gene Expression Related to Inflammation, Insulin and Lipid in Patients with Parkinson’s Disease: A Randomized, Double-blind, PlaceboControlled Trial

Shokoofeh Borzabadi 1, Shahrbanoo Oryan 1,2 * , Akram Eidi 1, Esmat Aghadavod 3, Reza Daneshvar Kakhaki 4, Omid Reza Tamtaji 5, Mohsen Taghizadeh 3, Zatollah Asemi 3

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Biology, Faculty of Science, Kharazmy University, Tehran, I.R. Iran.
3 Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran
4 Department of Neurology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
5 Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran
*Corresponding Authors: Shahrbanoo Oryan, PhD; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Department of Biology, Faculty of Science, Kharazmy University, Tehran, I.R. Iran. Email: Sh_oryan@khu.ac.ir Zatollah Asemi, PhD; Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R. Iran. Tel: +98- 31-55463378; Fax: +98-31-55463377, Email: asemi_r@yahoo.com

Abstract

Background: This study was conducted to evaluate the effects of probiotic supplementation on gene expression related to inflammation, insulin and lipid in patients with Parkinson’s disease (PD).

Methods: This randomized, double-blind, placebo-controlled clinical trial was conducted in 50 patients with PD as a pilot study. Participants were randomly allocated into two groups to take either 8×109 CFU/day probiotic supplements or placebo (n = 25 each group, one capsule daily) for 12 weeks. Gene expression related to inflammation, insulin, and lipid was quantified in peripheral blood mononuclear cells (PBMC) of PD patients, with RT-PCR method.

Results: After the 12-week intervention, compared with the placebo, probiotic intake downregulated gene expression of interleukin-1 (IL-1) (P = 0.03), IL-8 (P < 0.001) and tumor necrosis factor alpha (TNF-α) (P=0.04) in PBMC of subjects with PD. In addition, probiotic supplementation upregulated transforming growth factor beta (TGF-β) (P = 0.02) and peroxisome proliferatoractivated receptor gamma (PPAR-γ) (P = 0.03) in PBMC of subjects with PD compared with the placebo. We did not observe any significant effect of probiotic intake on gene expression of low-density lipoprotein receptor (LDLR) and vascular endothelial growth factor (VEGF) in PBMC of patients with PD.

Conclusion: Overall, probiotics supplementation for 12 weeks in PD patients significantly improved gene expression of IL-1, IL-8, TNF-α, TGF-β and PPAR-γ, but did not affect gene expression of VEGF and LDLR, and biomarkers of inflammation and oxidative stress.

Cite this article as: Borzabadi S, Oryan S, Eidi A, Aghadavod E, Daneshvar Kakhaki R, Tamtaji OR, et al. The effects of probiotic supplementation on gene expression related to inflammation, insulin and lipid in patients with Parkinson’s disease: a randomized, double-blind, placebo-controlled trial. Arch Iran Med. 2018;21(7):289–295.
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