Arch Iran Med. 2018;21(9):387-392.
PMID: 30221528
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Original Article

Hypericin Induces Apoptosis in MDA-MB-175-VII Cells in Lower Dose Compared to MDA-MB-231

Niusha Abbasi Gamasaee 1, Maryam Radmansouri 1, Saeedeh Ghiasvand 2, Fatemeh Shahriari 3, Hadi Zare Marzouni 4, Hoda Aryan 5,6, Ehsan Jangholi 6,7, Mohammad Amin Javidi 1,3,8 *

1 Department of Molecular and Cellular Science, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
2 Departments of Biology, Faculty of Science, Malayer University, Malayer, Iran
3 Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
4 Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
5 Young Researchers and Elite Club, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
6 Medical Students’ Scientific Association (MSSA), Tehran Medical Sciences Branch, Islamic Azad University Tehran, Iran
7 Clinical Research Development Center, Amir-almomenin Hospital, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
8 Cancer Alternative and Complementary Medicine Department, Breast Cancer Research Center, ACECR, Tehran, Iran
*Corresponding Author: Mohammad Amin Javidi, PhD; Department of Molecular and Cellular Science, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran-Iran (IAUPS), Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran, 8Cancer Alternative and Complementary Medicine Department, Breast Cancer Research Center, ACECR, Tehran, Iran. E-mail: Email: maj136@ gmail.com

Abstract

Background: Breast cancer is the major cause of death from cancer among women around the world. Given the drug resistance in the treatment of this disease, it is very important to identify new therapies and anticancer drugs. Many studies demonstrated that hypericin could induce apoptosis in different cancer cell lines; however, the underlying mechanism is not well understood yet. Therefore, this study aimed to evaluate the anticancer effect of hypericin in two breast cancer cell lines, one with wild type P53 and the other with mutant P53.

Methods: In this study, the MDA-MB-231 and MDA-MB-175-VII cell lines were treated with different concentrations of hypericin for 24 and 48 hours. The measurement of cell death was performed by MTT assay. The cell apoptosis rate was measured using annexin V/propidium iodide assay through flow cytometry. The level of expression in P21 and P53 genes was evaluated by real time PCR. Immunocytochemistry (ICC) analysis was performed for P21 (direct target for P53 protein) to confirm the results.

Results: The results showed that hypericin could have dose-dependent cytotoxic effects on the MDA-MB-231 and MDA-MB-175- VII cell lines, and its cytotoxicity is much higher in the latter cells. According to flow cytometry results, 86% of MDA-MB-175-VII cells underwent apoptosis with IC50 dose of hypericin for MDA-MB-231 cells after 24 hours. Moreover, after 24 hours of exposure to hypericin with MDA- MB-231 IC50 concentration, the expression of P53 and P21 genes upregulated in MDA-MB-175-VII much more than MDA-MB-231 when both cell lines were treated with 24 hours IC50 dose of MDA-MB-231. The ICC analysis on P21 confirmed that by treating both cell lines with MDA-MB-231 IC50 dose of hypericin for 24 hours, this protein is overexpressed much more in MDA-MB-175-VII cells.

Conclusion: The results of this study demonstrated that hypericin’s apoptotic and cytotoxic effects on cancer cells may be mediated via P53 overexpression, cell cycle arrest and the subsequent apoptosis. Therefore, it is of great importance to consider that hypericin would have better impact on cells or tumors with wild type P53.

Cite this article as: Abbasi Gamasaee N, Radmansouri M, Ghiasvand S, Shahriari F, Zare Marzouni H, Aryan H, et al. Hypericin induces apoptosis in MDA-MB-175-VII cells in lower dose compared to MDA-MB-231. Arch Iran Med. 2018;21(9):387–392.
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